| 1 | Sioux Falls School District 49-5 | Sioux Falls | 25,216 |
| 2 | Rapid City Area School District 51-4 | Rapid City | 12,432 |
| 3 | Harrisburg School District 41-2 | Harrisburg | 6,098 |
| 4 | Brandon Valley School District 49-2 | Brandon | 4,991 |
| 5 | Aberdeen School District 06-1 | Aberdeen | 4,263 |
| 6 | Watertown School District 14-4 | Watertown | 3,690 |
| 7 | Brookings School District 05-1 | Brookings | 3,446 |
| 8 | Yankton School District 63-3 | Yankton | 3,086 |
| 9 | Meade School District 46-1 | Sturgis | 3,034 |
| 10 | Huron School District 02-2 | Huron | 2,935 |
| 11 | Douglas School District 51-1 | Box Elder | 2,753 |
| 12 | Mitchell School District 17-2 | Mitchell | 2,711 |
| 13 | Pierre School District 32-2 | Pierre | 2,688 |
| 14 | Tea Area School District 41-5 | Tea | 2,408 |
| 15 | Spearfish School District 40-2 | Spearfish | 2,387 |
| 16 | Todd County School District 66-1 | Mission | 2,049 |
| 17 | Oglala Lakota County 65-1 | Batesland | 1,853 |
| 18 | Vermillion School District 13-1 | Vermillion | 1,432 |
| 19 | West Central School District 49-7 | Hartford | 1,409 |
| 20 | Dakota Valley School District 61-8 | North Sioux City | 1,386 |
| 21 | Belle Fourche School District 09-1 | Belle Fourche | 1,296 |
| 22 | Lennox School District 41-4 | Lennox | 1,224 |
| 23 | Madison Central School District 39-2 | Madison | 1,178 |
| 24 | Milbank School District 25-4 | Milbank | 1,014 |
| 25 | Dell Rapids School District 49-3 | Dell Rapids | 973 |
| 26 | Sisseton School District 54-2 | Sisseton | 970 |
| 27 | Tri-Valley School District 49-6 | Colton | 932 |
| 28 | Custer School District 16-1 | Custer | 906 |
| 29 | Wagner Community School District 11-4 | Wagner | 900 |
| 30 | Hamlin School District 28-3 | Hayti | 881 |
| 31 | Canton School District 41-1 | Canton | 880 |
| 32 | Chamberlain School District 07-1 | Chamberlain | 848 |
| 33 | Winner School District 59-2 | Winner | 825 |
| 34 | Hot Springs School District 23-2 | Hot Springs | 739 |
| 35 | Sioux Valley School District 05-5 | Volga | 715 |
| 36 | Flandreau School District 50-3 | Flandreau | 698 |
| 37 | Elk Point-Jefferson School District 61-7 | Elk Point | 690 |
| 38 | Beresford School District 61-2 | Beresford | 672 |
| 39 | Lead-Deadwood School District 40-1 | Lead | 671 |
| 40 | Groton Area School District 06-6 | Groton | 601 |
| 41 | Mobridge-Pollock 62-6 | Mobridge | 591 |
| 42 | Bon Homme School District 04-2 | Tyndall | 580 |
| 43 | Baltic School District 49-1 | Baltic | 580 |
| 44 | Parkston School District 33-3 | Parkston | 569 |
| 45 | Webster Area 18-5 | Webster | 548 |
| 46 | Deuel School District 19-4 | Clear Lake | 544 |
| 47 | Chester Area School District 39-1 | Chester | 542 |
| 48 | Redfield School District 56-4 | Redfield | 534 |
| 49 | Platte - Geddes School District 11-5 | Platte | 509 |
| 50 | Hill City School District 51-2 | Hill City | 508 |